I want to study naive CD4 T cells polarization and therefore I need to be sure that the cells I will culture will be naive. So far I have found isolating kits from Miltenyi, STEMCELLS Technologies and R&D. Does anyone else use a different kit?
Do you remember the percentage of naive cells purity? Would you recommend the Miltenyi kit? I think about that option because I dont want to torture my cells through FACS.
I have used the CD4+ CD62L+ kit from Miltenyi to isolate naive CD4 from mouse spleen and it works well. I would look to see the number of CD62Llo cells in your lymph node prep to see if you could just isolate CD4+ T cells without having to use the more expensive CD4+CD62L+ kit.
Yes I see. I would sleep quietly if that kit used a anti-CD44 for the isolation, but still (based on the datasheet) I think it would be reliable. I will check what you said. Thanks!
did you loo at RND syst kit? They usually have nice stuff.... http://www.rndsystems.com/pdf/magm205.pdf
There is a new magnetic bead separation technology that works very well - better than Miltenyi. See it at http://www.biocapturellc.com Not expensive.
@Jonas Cicenas. Yes I did but we would need to buy the ecquipment as well and we can't afford it at the moment. So, I'm looking for cost-effective options.
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