Aflatoxin B1 is soluble in organic solvent like Chloroform, Benzene, Methanol, Acetonitrile etc. But after time it may loose quantity. Also temperature and light are degrading aflatoxin B1. Stability depends upon exposure time of light.
I agree with the previous answer, light significantly affects aflatoxin stability in solution. According to my experience methanol is a good solvent and aflatoxins (B1, B2, G1 and G2) are stable for months if you dissolve them in methanol and keep in an amber vial at -20°C or, at least at 4°C. If you dissolve aflatoxins in a methanol: water mixture 50:50 v/v, the stability is significantly affected (no more than 1 month even at 4°C).
Alessandra Ricelli Hello, can you provide me any journal with that info thank you.
Actually, I verified what I wrote through my experience, however the study indicated could be useful
Diaz GJ, Cepeda SM, Martos PA. Stability of aflatoxins in solutions J AOAC Int. 2012 Jul-Aug;95(4):1084-8.
Alessandra Ricelli Great! Thank you very much for your help :)
This is true, stability of these mycotoxin is an issue. As per my previous experience, I agree with Alessandra Ricelli answer.
Hello Alessandra Ricelli, Could you tell me the max solubility of aflatoxin B1 in methanol? In my lab we are using DMSO as a solvent but by doing so we can obtain a stock solution with a maximum concentration of 36mM. We are hoping to achieve a higher-concentration stock by using methanol instead of DMSO. Thank you in advance!
Dear Irene, you are right, the solubility limit for afB1 in DMSO is about 36mM. Unfortunately, I do not know the solubility limit for afB1 in methanol and I could not find any article or study that specify this aspect. I think that the solubility in methanol should be higher than in DMSO. It could be worth to use a mixture of methanol:water 80:20 to improve the solubility of the toxin.
I'm sorry but I don't understand why AFB1 is dissolved in DMSO ?
Alessandra Ricelli
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