Do you have in your country a regulation on sewage sludge in agriculture?

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How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

Does anyone have a qPCR protocol for Lactuca Sativa?

I need to extract RNA, produce cDNA and do my measures via qPCR. How much material? Which stage of growth?

24 January 2021 559 2 View

Is PROCESS v3.5 by Andrew F. Hayes compatible with SPSS v 26 or 27 on Windows?

I installed the PROCESS Custom Dialog v3.5 by Andrew F. Hayes on two different versions of SPSS (26 and 27, both on Windows 10) running SPSS as an administrator and using the 'extension' menu....

30 November 2020 1,390 3 View

What are the digital competencies that university professors/trainers need to perform well & answer the demands of digital transformation businesses?

I would like to have access to academic research that compares the skills needed by university professors and corporate education instructors or facilitators. I would like to understand if the...

29 November 2020 442 3 View

Is there any research on in-vitro meat about sporadic cancerous cells and their possible impact?

The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?

10 September 2020 3,666 1 View

Which is the best transfection method for cell lines that growth in suspension?

We are trying to transfect lymphoma cell lines, and we have tried several methods, the best is through the use of lentivirus of course, but in this pandemic days is very difficult to import this...

06 July 2020 8,043 12 View

Social media_systematic participation to giveaways?

I am analysing the outcomes of a pilot project involving people' reaction (reaches and engagements) to posts related to plastic pollution. To enhance engagement we proposed "challenges" i.e....

10 November 2019 571 0 View

Problems with RT-PCR of respiratory syncytial virus. Any advise?

Hello! I am having difficulty amplifying the coding sequence of RSV F protein. The protocol I have used is a sfollows: I infect HEp-2 cells with the virus, then when CPE is evident I collect the...

02 October 2019 3,061 3 View

Where does a single nucleotide ambiguity in sequencing of a digested plasmid, that was not in the original plasmid, come from?

We have been having issues with this certain plasmid for a while: we were trying to remove one gene (GFP) and replacing it with another. The digest of the original plasmid with BamHI and NotI...

11 August 2019 8,202 8 View

Is anyone working with phosphorylated-S6K antibodies?

I am currently working with mTOR inhibitors and assessing their effect through the phosphorylation of S6K, but apparently the antibodies I am using are not specific enough. I am attaching an...

02 May 2019 7,504 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Which is the most ecofriendly and effective activated agent for the preparation of activated carbon from sewage sludge?

I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...

07 February 2021 9,507 5 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View