Do you have a protocol for biotinylation of the protein?

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Adam Hughes

The "instruction" documents for NHS-Biotin and its other derivatves give a general protocol for biotinlation based soley on the molecular weight and concentration of the protein in solution.

http://www.piercenet.com/product/ez-link-nhs-biotin

Neeraja Venkateswaran

We use NHS-Biotin regularly for biotinylation in a molar ration of 1:25 and get very good results. It is a very simple protocol. I can send you a detailed protocol if you send me an email at [email protected]