Do you consider that the serum transglutaminase is enough sensible for suspecting the diagnosis of celiaca disease?

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

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Anyone that can help with details in phosphopeptides enrichment?

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28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

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23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

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22 February 2021 4,352 2 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

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21 February 2021 7,457 3 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

Wesam Salah Ibrahim

There are two blood tests to help diagnose celiac disease:

1- Serology testing looks for antibodies in the blood. Elevated levels of certain antibody proteins indicate an immune reaction to gluten as tTG-IgA Test.

2- Genetic testing for human leukocyte antigens (HLA-DQ2 and HLA-DQ8) can be used to rule out celiac disease.

But the definite diagnosis by colonscopy and biopsy.

Ruediger Buchkremer

I have found a paper dedicated to this question and it claims that it is not enough. https://www.gastrojournal.org/article/S0016-5085(17)35624-X/fulltext?

Dear Dr. Rodrigo, thank you very much. I just did it for fun ...with Artificial Intelligence. I am very happy that I could help.