Good day. I have difficulty applying my data set to a Machine Learning Clustering algorithm, can anyone assist me in this regard?

I have a data set for a railway wagon: It consists of 9 measurement parameters per wheel (hollow wear, flange thickness, etc) and there are 8 wheels on a wagon. I need to work out a health index...

23 June 2020 838 11 View

How do I change category from an article to a book?

Several books have been listed as articles.

16 April 2020 6,631 3 View

How to specify accurately the Parameters for the slope and intercept when using Latent Growth Modelling?

The usual procedure when using a Latent Growth Modeling with, for instance, 4 time points, is to code the intercept T1=1, T2=1, T3=1, T4=1 and the slope T1=0, T2=1, T3=2, T4=3. From my...

14 February 2020 7,172 6 View

The astigmatism of the eye ==> some quantic problems. Ideas and experiments ?

The astigmatism of the eye ==> some quantic problems. No one photon can be 5 m wide on the mirror of the Mount Palomar, simply because the geometrical defects of the mirrors limit the size of...

29 January 2018 1,475 4 View

Does soil fertilization impact differently on shoot growth in trees and on the number of leaves on these shoots?

Is it possible that fertilization of trees by spreading N-P-K on soil brings to a longer annual shoot growth but in an other hand they will have less leaves each?

25 November 2017 9,852 22 View

Do you know the locality of "Ain Mauddud" in Qatar?

The 1975 lexicon of Qatar states that the Cretaceous Mauddud Formation got its name from that locality near Dukhan.The name was assigned by Dr. F.R.S. Henson in an unpublished 1940 report. Does...

30 July 2016 6,048 6 View

What is the best marker of human kupffer cells in immunohistochemistry?

I'd like to make an immunohistochemistry on human samples and find the kupffer cells. How can I do that? Thank you!

17 January 2016 7,574 3 View

Anyone familiar with rearing locusts (Schistocerca gregaria) in great quantity?

06 February 2015 6,753 4 View

What are the assumption of equal spacing of the repeated factor in repeated-measure ANOVA?

In repeated measure-analysis of variance (univariate, multivariate or mixed models), when time is the repeated factor, do times have to be equally-spaced? Any citable reference would also be...

13 August 2013 9,051 1 View

Looking for validated qPCR primers for estrogen receptors (esr 1 and esr2) in rat

In order to determine the effects of endocrine perturbators in RAT cells from colon tumors, I want to determine their impact on mRNA expression of esr 1 and 2 . Due to the low level of esr 1...

30 October 2012 2,743 7 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View