Do we need to include all fish species in constructing phylogenetic tree? Whats the minimum number of species that can be included in Phy. tree?

More Maria Mojena Gonzales Plasus's questions See All

Is it possible to get less than 1 FCR?

I am using a juvenile fish for my experiment. My FCR seems to be lower than 1. I heard once in an International conference that it is not possible to get less than 1 FCR.

09 October 2019 7,142 8 View

What is the difference between absolute and relative gene expression level? When do we usually use it?

I am doing a q-PCR for the gene expression level on different organs in common carp. I usually use beta-actin as my housekeeping gene and the delta CT value is around 16-18. In analyzing my data I...

01 February 2018 7,477 3 View

Can anyone suggest a journal that had fast review process the scope of which including but not limited to fish biochemistry and molecular biology?

We have limited time to publish our study as one of the requirement for graduation.

31 December 2017 1,707 0 View

Does anyone here work on taurine synthesis in fish? What is the latest findings about taurine synthesis in fish?

I read this article.

01 February 2015 9,118 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

Abhishek Kumar

what is your research question, that decides what to add and what to not add,

if you are doing vertebrate wide phylogenetic trees, than there are more 10 genomes of fishes, which you can add in this analysis

Mustafa S. Faddagh Ziyadi

I think it is suitable to include all samples belong to the studied clade(s) while out-group would beneficial to rearrange the sample genome in the genetic relationship tree