Dear Abbasi,

Rota evaporator only helps to concentrate your bio-active extracted molecules present in your medicinal plant. Hence, its quite beneficial to detect the single molecules exhibited in a extremely low quantity. Secondly, u have to lyophilize your extract after rota-eva. so that further analysis can be done without deteriorating the bioactives present in your extract.

Hope so it will help

Dear Abbasi,

In addition answer above mentioned by Dadwal, you can use it to evaporate solvent at low temperature protecting thermal unstable compound. However, If you want evaporate water using a rotary evaporation, you have to cool the solvent collecting vessels (using Ice) while maintaining, temperature of plant extract around 30-40 degrees of Celsius (but you have to be careful about the temperature). Since water is a polar solvent, it will take relative longer time to evaporate it

However if, your target compounds are highly volatile , you have to think about other extraction method.

Piyal Aravinna

Very good answers are given by Dr. Dadwal and Dr. Aravinna.

I just one to add one thing. Why are you not thinking about analysis of extract of succulent part of medicinal plants?

If you feel interest, you may visit my RG site for relevant articles.

Thanks.

Thank you Dr Vikas Dadwal and Dr. A. G. Piyal Aravinna for your insights.

Dr. Shibabrata Pattanayak my project is based on using the parts of plants that have high quantities of antioxidant and anti-inflammatory metabolites. for some plants its the leaves, for some its the roots and bark and so on. Currently im working on leaves.

Thank you.

Dear Dr. Abbasi,

You are working on leaves of some medicinal plant.

You are evaluating extract of DRY leaves.

Why are you not including SUCCULENT leaves as an extra parameter ?

It may be more effective as a medicine as it contains many other phytochemicals than dry one.

Our ultimate target is to benefit human society. Is not it?

I am agreed with Dr. Shibabrata Pattanayak , metabolites extraction of medicinal plant is different from others and its starts deteriorate from the time of plucking. Hence, people used to put it directly into liquid nitrogen without wasting too much time. And then crush it in lab for further analysis without drying or something. Medicinal plants metabolites are highly susceptible to light intensity, high temperature, pH even time. So, its better to made extraction from the fresh ones or use SUCCULENT leaves as advised.

Dr Vikas Dadwal are you suggesting that I eliminate the air drying step completely? If you have protocol for extraction from fresh plant material, I'd be happy to try it in my lab.

Thank you.