Due to the differences in the reaction mechanism of various antioxidant assays, it is recommended to conduct multiple antioxidant assays during antioxidant activity evaluation to come up with a good overall picture of the antioxidant potential of a given plant extract. Is there a minimum number of antioxidant assays that should be conducted in antioxidant activity evaluation? Which in vitro antioxidant assay can best mimic the in vivo model?
Antioxidant methods such as DPPH,· ABTS·+, nitric oxide, super oxide,LPO, metal chelating confirming the free radical scavenging property of the plants.
The stability of the free radicals in those reagent should be a a determining factors in chosing the best antioxidant assay...This stability is presented by DPPH & ABTS free radicals...
Yes, there is a number of in vitro assays are available which could use to assess the antioxidant activity. A good number of research articles published, so far, for evaluating antioxidant activity of various samples of research interest by different methods like DPPH method, Hydrogen peroxide scavenging (H2O2) assay, Nitric oxide scavenging assay, Peroxynitrite radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC) method/ABTS radical cation decolorization assay, Total radical-trapping antioxidant parameter (TRAP) method, Ferric reducing antioxidant power (FRAP) assay etc.
In my opinion, DPPH free redica assay,ABTS and Trolox equivalent antioxidant capacity (TEAC) may give you satisfactory results regarding the antioxidant activity.
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