Which approach do you use to determine the limit of detection for ELISA data?

Several approaches are used for the determination of detection limit in analytical chemistry. I wonder which is the best approach for ELISA data.

04 May 2019 9,087 3 View

How to recover plasmid DNA from filter paper?

I got a couple of plasmids on filter paper. Does anyone have a protocol to extract plasmid DNA from filter paper?

09 October 2014 4,476 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

No title

23 February 2021 4,014 3 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

What is the possibility of development of vaccine against plant disease ?

Vaccination has been extensively used to prevent, eradicate and control infectious diseases. If it can be made for plant its process will be ....but can it be on progress .

21 December 2020 3,420 15 View

No title

I am medical student. I want robbins pathologie fr deutsch pdf . how am I find this book ?

13 December 2020 8,136 3 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View

Is there a difference in the effect of temperature on the pathological and isolated Klebsiella from the environment?

I want to know the effect of temperature on the growth of bacteria, as well as on the ability of bacteria to nitrogen fixation.

22 November 2020 3,623 2 View