Previously, I colllected the oral swab samples in Zymo DNA/RNA sheild solution. I would like to perform the saponin based host depletion before doing the shotgun metagenomic sequencing.
I have concerned that DNA/RNA sheild could break the bacterial cell prior to host depletion. I have no idea about the component of this reagent. Do anyone have suggestion about this concern?
This solution is for preserving and freezing (as in time terminology) the physiological condition of cells, irrespective if they are eukaryotic or prokaryotic. I do not know how would you deplete host cells, but that is different issue. This solution should not cause or cause minimal harm to cells.
We have used this with mycobacteria and they remain in tact. The purpose is to preserve the cells and inactivate them, without lysis
It will not break but please do not leave it long time otherwise you will lost some.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases