Is it normal for the SHSY5Y cells to look this way post thawing from Liquid Nitrogen? do they have any chance of recovery?
N.B. Using the same procedures but within the -80C storage, they were perfectly fine.
The appearance of SH-SY5Y cells post-thawing from liquid nitrogen suggests they may be experiencing stress or damage. Here are some key observations and recommendations:
Key Observations:
Recommendations for Recovery:
Despite the initial stress, SH-SY5Y cells have a chance of recovery with proper care. Monitor their progress over the next 48 hours. If no improvement is seen, consider revisiting your cryopreservation protocol.
Un update on this, there has been a problem with the media. The SHSY5Y cells should recover from liquid Nitrogen in a similar morphology to -80C if exact thawing procedures are followed with adequate media (DMEM/F12, 1% pen/strep, 1%NEAA and 10% FBS).
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