Dina Ahmed

5 Questions 19 Answers 0 Followers

Questions related from Dina Ahmed

Do the SHSY5Y cells look weird post thawing from Liquid Nitrogen?

Is it normal for the SHSY5Y cells to look this way post thawing from Liquid Nitrogen? do they have any chance of recovery? N.B. Using the same procedures but within the -80C storage, they were...

19 March 2025 2,509 3 View

SHSY5Y cell culture- is that black dot or fungal contamination?

Can anybody performing SH-SY5Y differentiation confirm whether these particles-which appeared at day 3/4 post adding retinoic acid- are fungal/black dot contamination, dust particles or cell...

12 August 2024 8,181 6 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

02 July 2024 8,844 3 View

Human neuronal cell line used for cell migration assay (scratch injury specifically)?

Is there a human neuronal cell line used as an in vitro model to apply scratch injury to perform cell migration assay? Other than SHSY5Y as I am using it already. If any, any special hints about...

21 May 2023 7,497 6 View

What is the most applicable protocol for SHSY5Y cells differentiation?

If I am using the SHSY5Y cell line to test CNS regeneration, do I have to differentiate it to be more reliable? if yes, what is the most reliable & applicable protocol? Also, any hint on the...

07 May 2023 9,682 15 View