What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

How to perform a meta-analysis of median and 95% confidence interval?

i am perform a meta-analysis, some published studies provide median and 95%CI of hospital stays, according to some search, i found two ways to pools median data: one is use excel or web-based...

24 August 2019 1,770 3 View

Ways to suppress radiative thermal conductivity?

Dear all, With increasing temepratures, the radiative thermal conduction gradually becomes a nonnegligible part of overall thermal conduction (i.e. lattice plus photon/radiative thermal...

01 November 2018 279 2 View

What is Pt-pressed electrodes in Impedance measurement?

In the paper Superprotonic Conductivity of a UiO-66 Framework Functionali... , the authors mentioned that they used " a home made sample holder composed of Pt pressed electrodes". Anyone know...

08 September 2018 8,257 1 View

What is the recommended practice for analyzing Flow Cytometry cell cycle data?

I am analyzing cell cycle data from flow cytometry using PI as a single dye, and I encountered several questions: (1) Which signals should be used for gating the cells? Some say FSC-SSC, others...

28 May 2018 6,788 2 View

How do I make ITO electrodes on an organic-inorganic hybrid crystal?

12 April 2015 2,662 1 View

How do I make ITO electrodes on an organic-inorganic hybrid crystal?

03 April 2015 2,717 1 View

How can one design solid state reaction experiments?

Many compounds, most high temperature superconductors for example, were got by solid state reaction. How did researchers design such experiments?

11 December 2013 4,065 3 View

Separation and purification of DNA isoforms

I have a sample of long chain DNA (~ 100 kb), and it is a mixture of different isoforms (supercoil, relaxed circular, nicked circular, and linear). But I only want relaxed circular from the...

27 December 2012 642 1 View

How to concentrate DNA solutions?

I am having some DNA solution of 1 mg/ml, and I want to concentrate the solution to 10 mg/ml?

10 November 2012 8,656 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View