I have counted numbers of individual of different species which have divided into eight different phenological states. Now I want to calculate diversity indices, should I need to add all stages of phenology of given date?
No, just count the species independent of phenological state :)
For calculating diversity indices, you do not need to consider any phenological stages. Just count the number of species. But you did a great job. If you have any treatment, then you can test the effects of your treatment on phenological shift. Good luck.
Dear all respected repliers @ Andrew Paul McKenzie Pegman , Subir Bandyopadhyay and Mohammed Abu Sayed Arfin Khan ,
Thank you very much for your valuable reply. Here, I have attached an example of a phenological data format which I want to calculate diversity indices of it. Can you suggest me which column should I use as a species number, either B, C...and I or total of all in each date?
In the species column, always one (1) is written. What does this mean? I guess you did an experiment with single species (monoculture) and counted responses of its several individuals? am I right? If not, please explain your experiment. We can then come up with an idea.
Dear Mohammed Abu Sayed Arfin Khan
No, this is only an example, I have more that 20 species with same format with 60 plots.
To measure the diversity indices, you just need the total number of species found in a plot (out of 20 species). But, in the excel file the column "total" indicates the total number of individuals of a species located in a plot.
Dear Mohammed Abu Sayed Arfin Khan
Thank you very much for your valuable reply.
Dahal
Yes Digamber for calculating diversity indices u need to found total number of species found per plot and than total. Phenological stages are to see the differences and pattern.
To calculate species richness, all you need is to count the number of different species. To calculate diversity indices, you need the abundances of each species as well, i.e. how many individuals of each species was in each plot. Given your data, you could look at it in multiple ways. To quantify overall diversity, totals are combined across all sampling dates for each species. But you might also be interested in seeing if diversity changes over time, e.g. diversity per each sampling date. I suggest EstimateS, which is a free software program you can download. You would need to create a separate spreadsheet for each plot. Usually there are more species than sampling dates, so it generally makes more sense to have columns be the different sampling dates and rows be the species. Each cell of a row would be the total individuals of that particular species collected/observed on that particular sampling date. If I understand the data you sent, for species 1 you would enter the final column. Then you'd do the same for species 2, 3, etc. You can enter as you have, with columns being rows and species abundances being columns, but it just means you'll have a wide spreadsheet that will require a lot of scrolling width-wise if you have a lot of species. I find it easier to scroll down, versus across. EstimateS calculates multiple diversity indices and will give a value based on the first sample all the way up to the total number of samples. Plus, it generates species accumulation curves and non parametric measures of total estimated richness. It's pretty user friendly, and also has a guide.
Dear Janice Bossart
Thank you very much for your valuable reply.
Dahal
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