Do anyone know a nonionic detergent that can be sterilized by UV? My experience is that Triton X-100 will be degraded if UV irradiated.
Googeling this will only lead to threads where people say there is no need to UV irradiate Triton since nothing can grow in there. This is true in a way, but if you work with ultra low input techniques and have DNA floating around in your Triton it might cause a lot of noise in your data.
I am going to lyse microbial eukaryotes.