Hi fellow researchers,
I'm looking forward to conducting an experiment in which I'd like to irradiate a substance in an aqueous buffer solution (pH = 7,7) containing 5 % of DMSO. My goal is to investigate the substances behaviour when being exposed to UV-light. Iradiation time should expand from several hours to several days.
Does anyone know if DMSO degrades in some kind of polymeric or other influencing way which might distract my frequent HPLC-analytics?
I've found a paper in which the authors investigated degradation pathways of DMSO under UV-Irradiation to small organic molecules (e.g. dimethylsulfide) which should not distract HPLC-analysis by producing strongly UV-absorbing by products:
https://www.researchgate.net/post/Is_DMSO_light_sensitive
Best regards,
Ferenc
Sodium is a potential issue, so if you can avoid it in your buffer you would be better off. Dimerization or polymerization probably will not occur at that temperature, or at least not in my experience, especially in the presence of that much water.
My Buffer consist of Ammoniumhydrogencarbonate with calciumchloride as an additive.
have a look at this: Investigation on the stability of new biologically active thiosemicarbazone-derived compounds by a validated HPLC-PDA method
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