Hellow !

i'm about to work on a DMH induced colon cancer in mice; knowing that i can only work with either balbc or NMRI swiss mice :

1) what's the appropriate age to start the experiment and should i use male or female mice ? is there a significante difference ?

2) using a dose of 20mg/k once a week , how long does it take to have actual tumours and not just ACFs (precancerous lesions) ?

3) intraperitoneal or a subcutaneous injection?

Thank you .