Dear Simplisio

You are working with a yeast, that usually have high digestible energy. If the rest of the diet ingredients are not so digestible, it is possible that the GE of the whole diet could be lower than the DE of one particular ingredient. If you are working with in vivo digestibility, probably the best approach to determine P. pastoris digestibility, and calculate its DE, is to use a Latin square design with different levels of such yeast in the ration; take present that associative effects between feedstuffs is possible.

best regards,

Redimio

Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394841/

Also check https://jasbsci.biomedcentral.com/articles/10.1186/s40104-018-0254-0

https://www.mdpi.com/2076-2615/11/6/1696/pdf

Hello Sir,

You can try this method outlined in this paper

https://he.kendallhunt.com/sites/default/files/heupload/Jurgens_11e_Chapter4.pdf

Please check it.

https://www.sciencedirect.com/topics/food-science/metabolizable-energy

Please see this.

Article Determination and prediction of digestible and metabolizable...

Also check on this

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/metabolizable-energy

Dear Dr Emmanuel A Simplisio . See the following useful RG link: Article Digestible and metabolizable energy concentrations and amino...

Kindly see also the following very good link: https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/metabolizable-energy

The energy value of the feed is essential for formulating a balanced diet that meets the animal's need to continue to maintain and produce. Therefore, the DE value of the test ingredient in the diet is greater than the GE of the calculated diet. Pichia pastoris is used as a new feed material in the cattle diet due to its high content of Crude protein, since the DE value in Pichia pastoris is higher than the GE value of the processed diet.