Difficulty in bud break in Leptadenia reticulta?

03 March 2018 0 6K Report

I have tried to establish shoot cultures of L. reticulata using nodes as an explant, but since December no bud break was observed even in medium in which shoot formation was observed earlier. The explants were treated with PVP, bavistin and streptomycin (4 min each) which is also same as earlier.

What could be the reason and how to overcome it?

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