I would like to image differentiated Caco2 cells grown on a transwell and stained with fluorophore (ie Phalloidin -Alexa 488) without perturping the 3D morphology of the cells.

I have read that you can cut with a scalpel the filter and place it on a glass slide. I gess I should than add antifadding, but then? Should I add a coverslip? Will the coverslip not scratch out the apical surface of the CaCo2 cells?

Do you have a protocol to recommand? any suggestions are welcome