Determine the number of quails in a treatment/replication/pen?

How can we estimate the number of quail birds in a treatment and in a replication for 5 treatment and 4 replication for feed research? Is there any range limitation to keep the number of birds?

19 November 2020 2,856 3 View

Does any one know an integrated cloud governance framework for all levels of government (federal/state or provincial, and municipal)? Any suggestions?

How can the Social Cost Benefit-SCB Analysis be used in Cloud adoption? Perhaps, it is equally important as the Return on Investment-ROI?

04 September 2020 9,310 1 View

How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,132 12 View

Can you suggest some unconjugated primary antibodies for IHC against Pig formalin-fixed paraffin embedded(FFPE) tissue samples?

I am looking for unconjugated primary antibodies against CD3,CD4,CD8,CD11c, CD14, CD21,IgM, MHC-II in Pig FFPE tissue samples. Has anyone used Pig FFPE tissues for IHC against these markers ?

08 December 2019 1,450 3 View

Is it possible to perform Mann-Kendall Test for trend analysis of 5 years time series data?

I am having confusion and problem in trend analysis. Is it possible to perform Mann-Kendall test for trend analysis of 5 years time series data of groundwater level?

08 October 2019 733 4 View

Why PCR from cDNA is not working?

We are trying to clone a group of genes but failed at PCR step. What could be the possible reason? our protocol is briefly outlined below: 1. Extract total RNA from HEK293 cells (purity and...

07 May 2019 1,813 5 View

How is the processing of ERP signals done ?

I have collected 2 channels of EEG data with subjects looking at some visual stimulus. I have looked into a bunch of websites regarding EEG processing steps once we acquire the signal. But I have...

10 February 2019 5,989 5 View

Is there any oddball paradigm experiments written in matlab ?

I want to use oddball experiment for my EEG research. Can anyone provide any code which uses such oddball paradigm ?

10 February 2019 9,244 2 View

Is A4/Fuk cell lines metabolically active?

I have cultured A4/Fuk cell lines and it looked healthy in T75 flasks. I seeded for drug screening and growth curve. The cell count in growth curve in next day showed that there is no growth of...

06 February 2019 406 3 View

Can we do regression analysis in SPSS using the imputed data from measurement model in AMOS?

Dear Scholars I am going to test a complex model (11 latent constructs with 65 items). I did pooled measurement model in AMOS and reached good model fit with the help of modification indices. Out...

16 November 2018 6,347 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

In a random graph, the number of paths that cannot be extended in an hamiltonian path, can be roughly be separated in two equal parts ?

In a random graph, the number of paths that cannot be further extended in an hamiltonian path or an hamiltonian cycle, can be roughly be separated in two equal parts? I have an idea for solve for...

07 January 2021 9,782 2 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View