I need to stain adiposome in microglia, but I don't know how to confirm that are really success?

method :

Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at 4 degrees Celcius, removed and washed cells with ddH2O 3 times.

Remove and allow air to dry

Added 60 % isopropanol rinse and removed.

Oil Red O stock solution (1 % Oil Red O in 100 % isopropanol) and then filtered. Dilution of 3:2 (3 parts of oil red o, 2 parts of ddH2O), added stain to cells for 1 hour.

60% isopropanol rinse and removed.

Removed and washed wells 3 times with ddH2O.

And go to observe at light microscopy.

Look like it doesn't work.

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