Diagnostics applications for multiplex PCR using droplet microfluidics

More Drew Ramos's questions See All

Is it possible to manufacture polypropylene sheets using a biaxial process?

I know that films and other low-thickness laminated products are produced using biaxial orientation, but is there something similar for sheets with a thickness of around 6 mm? Or are they...

21 July 2024 1,559 1 View

My hippocampal mouse primary neurons clump when I plate them on microfluidic devices for axonal isolation. Any tip?

Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...

01 July 2024 8,070 0 View

What factors should be considered in developing a reliable serological test for HDV antibodies that can effectively account for the diverse genotypes?

Prior to the research carried out by the research group who published this journal, they assessed HDV RNA detection's diagnostic efficacy utilizing the HDV RNA detection technique and discovered...

11 April 2024 1,849 1 View

Does antigen retrieval for IF on tissue samples need to be done on the same day?

For both heat-induced or proteolytic-induced antigen retrieval, does the AR needs to be done on the same day of the IF? Or can be done one day in advance, store the slides at 4º, and performe the...

06 March 2024 220 1 View

¿qué es la narrativa de experiencia como método de investigación?

Necesito textos y estudios sobre el método de investigacio

17 February 2024 6,719 2 View

Patient with chest pain was taken to the emergency room, where the electrocardiogram presented was performed. I need help, as I am a medical student?

patient wakes up in the morning with retrosternal pain. dia 22 de fev THE PATIENT SEEKED THE CARDIO SERVICE AND PRESENTED THIS ECG. AND THE PROFESSIONAL REQUESTED THE FOLLOWING TESTS: 1...

19 December 2023 9,258 20 View

Bom dia , Poderiam me ajudar com este problema?

The Rules of Mixture give the stiffness in the fiber direction: EL=VfEf+VmEm For a composite with a fiber volume fraction of 55%, a glass fiber stiffness of 90 GPa, and a polymer matrix...

26 November 2023 6,967 0 View

Why is my plasmid larger after a single cut restriction enzyme digestion?

I have a 3.1 kb plasmid and I performed a digestion with the XmnI enzyme (37°C x 15 min and the enzyme was inactivated at 65°C x 20 min). When performing agarose gel electrophoresis, the size of...

21 November 2023 2,991 3 View

DFT adsorption of H on Ni(OH)2: correct conditions?

Hello, everyone I'm simulating on Quantum Espresso the H adsorption on Ni(OH)2 surface. However, upon relaxation of the system, H binds with one of the O atoms of the surface and forms water,...

31 August 2023 7,682 1 View

Does anyone have some reference about aquatic insects evolution?

I'm an undergraduate student in biology, and I study aquatic insect ecology with a focus on biodiversity and conservation, ecotoxicology, and trophic relationships. Scientific materials on the...

31 August 2023 6,641 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,666 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,952 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,032 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,078 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,743 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View