Determination of trehalose in wheat with HPLC

Faheem Ahmed Khan @Faheem_Khan3

05 May 2012 1 8K Report

I wish to know which is the best available way to determine trehalose concentration in wheat plant?

Robin Joshi

Trehalose is a disaccride sugar. You can determine using HPLC-ELSD detector.

Mobile phase ACN:Water, NH2 column. flow rate=0.8ml/min, column temp.=35C.

You will definitely get your results.

Gud luck

Badges
Science topic

More Faheem Ahmed Khan's questions See All

Wheat transformation

I know the best possible explant for transformation in wheat is immature embryos but the problem related to it is, it's too laborious and the time period to isolate it is too limited. Can anyone...

04 May 2012 9,507 6 View

Immunological studies with Characterized Natural Products?

It will be nice to do the immunological studies if the natural products you characterized have never been used before in autoimmune diseases.

01 January 1970 4,844 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,617 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,084 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,919 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,938 5 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,796 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,468 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,558 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,788 1 View