05 May 2013 0 7K Report

Does anyobody use a method of detection for histagged biomolecules in electrophoresis with terbium (lanthanide metal ion, which exhibits fluorescence) and dipicolinic acid (senzitizer, which enhances the fluorescence of the ion)?

More Maria Kamencayova's questions See All
Has anyone experience with detection of histidine tagged biomolecules on electophoretic gel by dipicolinic acid and terbium chloride?

A method for detection of his-tagged target biomolecule while said molecule remains in the gel in which it has been separated from other constituents by electrophoresis.

05 June 2013 471 3 View

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