Dear Professors, I have a question for pollen grain features, would you please help me?

More Atena Eslami's questions See All

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Is a wall with constant pressure and constant inlet flow similar to an inlet boundary?

At an opening in turbulent flow, using wall boundary condition, with constant flow rate and constant pressure inlet, would be similar to an opening with inflow BC?

03 February 2021 2,390 5 View

How to amplify specific pcr product?

Hello I try to amplify gene of interest by RACE-PCR. For 5` RACE, I saw multiple bands after using specific nested primer. But The desired band was not seen on the gel. What is the reason? When I...

31 October 2020 7,734 6 View

What is the best force field for proteins with folded and disordered regions?

Hello I want to do MD simulation on a protein model to refine its structure. My protein structure have both folded and intrinsically disordered regions. What force field should i choose for this...

23 August 2020 9,381 5 View

What is the best force field for protein MD simulation?

Hello I try to do molecular dynamic simulation with GROMACS software and I work on an enzyme in a plant producing a medicinal secondary metabolite. When i was searching to find a appropriate force...

31 July 2020 171 5 View

How to avoid smear from PCR product on agarose gel?

Hello I used high fidelity enzyme in my PCR and I saw smear on agarose gel. I repeated the PCR with different amounts of cDNA. But I did`nt get a sharp band. what should i do to resolve my problem?

29 July 2020 3,483 10 View

Which one is more important in protein homology modeling ?

I try to make a model with MODELLER software and I decide to use multiple template modeling method. Two template structure I choose for my work, have similar Identity percentage (42.54% and...

03 May 2020 1,263 3 View

How can i detect small size PCR product from primer dimer?

Do you know any method to distinguish small target band of primer dimer? ( They have similar size.) I tried higher concentration of agarose gel and longer electrophoresis run time. but i didn`t...

22 December 2019 387 9 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

Identify DNA bands transfer from gel to the membrane(southern blotting)?

Hello Is there a way to detect transferring of DNA bands from gel to membrane in southern blotting without staining? Thanks

10 November 2017 7,804 3 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Can anyone help in doing SEM of pollen?

Is there any institute in Kerala were v can do SEM studies.

11 November 2020 5,001 3 View

I need info on particular Convolvulaceae pollen, can you help?

Dear colleagues in palynology, paleobotany, and botany I desperately need information/papers on the pollen morphology of the following Convolvulaceae genera: Astripomoea, Calycobolus,...

14 October 2020 8,216 10 View

What is the best method for collecting and preparing pollen from insects stored in 70% ethanol, for visual identification?

Hello, I would like to visually identify and quantify the pollen morphotypes carried by insects which have been stored in 70% ethanol. The insects are Diptera (mainly Syrphids), and Hymenoptera...

20 August 2020 8,859 3 View

Is it possible to remove pollen from museum bee specimens?

I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...

25 May 2020 8,423 2 View

Can adult butterfly or moth feed on solid food, such as pollen?

Dear All, Can adult butterfly or moth feed on solid food, such as pollen? It is known that nectar mainly contains sugar but very low level proteins, lipids. If a butterfly only feeds on nectar,...

29 March 2020 6,211 3 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

How different do fossilised and fresh pollen look under the microscope?

I was wondering whether fossilised pollen looks very different from normal pollen when you use bright field microscopy? In particular, do the following change significantly: Morphology...

29 November 2019 1,269 9 View

When doing Scanning Electron Microscopy of pollen samples from basal angiosperm group, is it necessary to do acetolysis?

1. Will there be significant differences in SEM images of acetolysed pollen and pollen fixed in 70% EtOH? 2. This is to study the micro-morphology of pollen structure and ornamentation for...

10 November 2019 5,022 2 View

Is there a protocol to isolate germinated pollen from solid media for RNA extraction?

We are trying to isolate RNA from pollen of Phlox drummondii plant. We have cultured pollen on solid media where it germinates. We now want to isolate this germinated pollen to extract RNA. Does...

10 November 2019 500 1 View

Khadijeh Alinezhad

Dear Atena as far as I know, the best and thorough way to measure pollen grain is to use microscope.

Best

Khadijeh

Atena Eslami

Dear Khadijeh

So many thanks for your answer, but i know this and i used microscopic rulers. However, i want to know if there is any software to measure the distances or not.

Kind Regards

Atena

Samik Bhattacharya

Hi Atena,

l suggest taking a good resolution picture of the pollen structure with a microscope. Then use ImageJ software (https://imagej.nih.gov/ij/download.html) to analyze/measure the pollen structures. It is simple to use the software in manual mode just by drawing lines along the structure with the mouse.

Best,

Samik

Dear Samik

So many thanks for your nice answer. You solved my problem.

Best Regards

Richard G. Holloway

I have used the software which comes with the AMSCOPE family of digital cameras for the microscope. One calibrated, the researcher an measure lines, etc from the photograph and then the measurement is captured in an excel file. I have used this previously t measure characteristics in carbonized corn. I included a web address below

http://www.amscope.com/camera-download

so many thanks for your guidance.

Regards