Dear Guys I'm master student and want know a methods used for oligosaccharides in honeys which is simple ad effective way ? please react on it?

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Difo Voukang Harouna

Hi!

I think you should be able determine oligosacchrides in honey with the same methods used in determining them from plants or legumes. Nowadays, there several methods for that ranging from HPLC-RID/ELSD to TLC (Thin Layer Chromatography). You will need to extract the oligosaccharides using ethanol and led acetate.

Here are some useful article: Article Chemical Changes during Open and Controlled Fermentation of ...

Article Changes in nutrient and antinutrient composition of Vigna ra...

Regards!

You can also see this

https://www.researchgate.net/post/I_want_to_identify_and_quantify_oligosaccharides_in_honey_samples_Could_any_one_suggest_me_the_more_reliable_procedure

Roger A Laine

Use a BioGel P2 column to separate the glucose and fructose from oligosaccharides and concentrate them. Silica Gel G TLC with Acetonitrile:Water 4:1 to 6;1 should give you good separations of sucrose, raffinose, stachyose, etc. from the concentrated samples using authentic standards. A number of steps will be necessary if you want to characterize them completely.