How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Why are the organoid cells showing blue fluorescence even without dapi stain?

Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...

05 January 2021 2,067 3 View

Can anyone tell me the name of these parasites which I got from a marine fish?

I got these parasites from eye and intestine of a marine fish. Thank you.

09 December 2020 7,752 2 View

Is it alright to reuse the MinION flow cells using the same set of barcodes but for a different type of approach/targets?

It's generally recommended to use a different set of barcode when reusing flow cells due to issues of barcode carry over despite thorough washing of the flow cell when running another batch of...

05 December 2020 5,544 1 View

What is the best way to filter out cell debris/small particles from a cell prep?

Hello, I am isolating lamina propria cells from intestinal tissues and I would like to add an extra step at the end to filter out cell debris but preserve my cells. Should I use a 22um filter and...

14 October 2020 3,266 2 View

How to obtain good cryosections of mouse intestine?

I am attempting to do mouse intestine longitudinal cryosections, but the sections after hematoxylin and eosin staining are not at their best (the villi keep breaking apart). My protocol: I rinse...

14 October 2020 8,432 3 View

Can anyone help me in doing whole mount immunostaining on mouse colon?

I am exactly looking forward to know the procedure to isolate the myenteric and submucosal layer from intestinal whole mount. I would like to see the degeneration of dopaminergic neurons on it?...

12 October 2020 6,069 2 View

Why does chicken primary intestinal epithelial cell could not passage?

05 October 2020 454 3 View