I am planning to isolate bacteria from the rumen of cows to see if they have conjugated linoleum acid - activity. I read that rumen samples should be kept under anaerobic conditions and in cool conditions. Does someone know the best method to do it?
Hi Dr.
this link can help you
http://articles.extension.org/pages/28320/collection-and-preparation-of-milk-samples-for-microbiological-cult
Hello Dr.,
Please see the following information about Sample Guidelines
with link
http://www.idexx.co.uk/pdf/en_gb/smallanimal/reference-laboratories/UK_Sample_Guidelines.pdf
You can use the candle technique. You have to put your sample in a jar and light a candle inside the jar and seal its door.
Thank you to all of you for your answers. However I still miss the information to take rumen liquor samples.
Anaerobic condition can be maintained with Nitrogen gas..
use thermal container with carbon dioxide flashing only and quick transfer to the laboratory
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