Which kind of extraction of this plant was performed? Tell us more details, please. Which curcuma longa´s extracts have you assessed?

Dear Rafael lund

Thanks for the reply. sorry i forgot to mention. We tried with ethanolic, water and chloroform extracsof rhizome of curcuma longa at various dilutions 0.5mg/ml,1mg/ml,2mg/ml in DMSO using agar diffusion method, muller hinton agar,chocolate agar against S.mutans, MRSA, S.mitis, E.coli. conflicting reports in literature. we also noticed crude extract does not dissolve completely in DMSO or Tween 80. Water extracts were contaminated .can you please provide your valuable inputs?

I think that agar diffusion method has several problems for interpratation of halos results. Maybe you need to assess these extracts in microdilution techniques. I also use DMSO, Tween 80 or EtOH to dissolve my crude extracts. But, you need to try these extracts more soluble. You must to isolate their extracts´compounds and test, or try to become them more soluble in water reacting them with ciclodextrin, for example.

You should to consider photodynamic !! some vegetal extracts become very bactericidal after strong or specific light exposure.

In fact, I agree with you, Mr Codony. Some vegetable extracts can have this PDT effect.

Actually, we are also working with dynamic phototherapy against oram microorganisms like S. mutans, but with synthetic staining compounds.

Please, tell me about your experience with PDT.

Cheers,

Can you mention how do you prepare the extract... my opinion is to prefer the cold extract with methanol....

Mr Lund is a long to explain, but we have been using the systems form GenIUL for vPCR ( using photoreactive reagents ). Additionally some devices working with red and UV has been used for evaluate the effects of some psolarens over bacteria and yeast.

You can see some examples in

http://www.geniul.com/ca/products/instruments/phast-photodynamic.html

Sathesh,

Don´t you think MetOH is a toxic solvent?

We prefer use EtOH extact at a room temperature.

Cheeers!

Dear Lund

Methanol is a commonly used organic solvent that, because of its toxicity, can cause metabolic acidosis, neurologic sequelae, and even death, when ingested only. for complete extraction of phyto chemicals foe external application methanol is preferred.

also the minimal lethal dose of methanol in adults is believed to be 1mg/kg of body weight.

Ok, Sathesh. I have this problem of solubilisation of some extracts. It´s usual in my routine. And I still remain trying new techniques of solubilisation.

If I got new findings, I tell you about it.

Cheers!

Thank you, Francesc.

I really enjoyed this equipment. Is this expensive?

Thank you in advance.

Cheers!!

I think that not is expensive. Probably their price is lower than a good small microcentrifugue, but really devices like this are needed to support a good research line, for this reason I think that is cheap.

In some esential oils we use 20%DMSO as solvent

I m also working on curcumin nanoparticles.The best used solvent is DMSO.

Yes Satish kumar I got antimicrobial efficacy with cold methanolic extract agaist S.aureus

Thank you Bhavana. But we are facing problems in solubilising crude extract in DMSO. Should we keep it in gyromixer for 24 hours

hi Francesc Codony . I am facing problems with dissolving crude extract and essential oils in 20% DMSO. Do we need to keep in gyromixer for longer period of time?

please go through my paper on preparation ,characterization and antimicrobial activity of nanocurcumin.doi no. is dx.doi.org/10.1021/jf104402t.Hope the article will help you to some extent.

Really if your can't dissolve in DMSO I can't help you. I not have specific experience with your product. But is surprising. In the past I dissolved insoluble chemicals as Bismuth in BAL, ( 2,3-Dimercapto-1-propano) and sometimes using ultrasound baths.

Yes Fransec Codony. we could not get a homogenized solution of crude extract in DMSO by vortexing it. some of the residue sedimented below. This did affect the concentration of the solvent. May be I should try out ultrasound bath. but we dissolved it in respecive solvents like methanol and used it as control and we found antimicrobial activity against S. aureus.

Thanks Bhawana singh

Would anybody provide me a paper which contains the use of tween for solubilizing the extracts. i am trying to find it but i am not getting it...i tried a lot...thanks in advance..