15 October 2024 0 5K Report

La palabra ductal se refiere a que el cáncer empezó en los conductos lácteos

More Luciana Valeria Seminario's questions See All
Can I detect endogenous RNA in human plasma samples?

I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA...

22 May 2024 1,919 1 View

Does my Vero cells look contaminated?

Hi guys, We have been trying to get our cultures back after identifying contamination in our previous cultures. We have cleaned everything and started from uninfected cell stocks, but I’m not...

20 February 2024 6,717 0 View

How can I re-design a hydrolysis probe to avoid a non specific signal?

I am working on a multiplex qPCR to detect simultaneously a viral RNA target and an exogenous RNA internal control using hydrolysis probes. As you can see from the picture, this internal control...

01 February 2024 2,484 0 View

Yeast transformation confirmed in PCR but no protein expression ???

Hi everyone, I have been trying for months now to get my protein expression confirmed by western blot and failed every single time (I checked the antobodies, they are working fine). I have...

05 July 2023 6,935 5 View

Can you share wellbeing tips in marriage or romantic couples as a study outcome?

Hi All, I am writing about wellbeing tips in marriages or romantic couples and I need studies with the related outcome. Can you please share yours? Thank you. Alexandra

14 April 2023 1,905 1 View

How I decontaminaton Caco-2 cell line?

Hello everyone I have been working with the Caco-2 strain for a few months, but unfortunately, the cells were already contaminated. I already tried to decontaminate with penicillin/streptomycin...

04 April 2023 849 1 View

How is the variability of eletronic noses of the same brand?

Hello, someone who has already worked with electronic noses such as Cyranose-320 knows if there is variability between different devices of the same brand? Thank you Luciana

05 December 2022 3,042 1 View

Is there a recommended percentage of a fermenter volume that a cooling coil should occupy?

I've been working on the heat exchanger design for fermentation systems, and found an explanation in the literature on how to calculate the cooling-coil length. However, I haven't found...

17 February 2022 8,660 4 View

Could someone help me withAlizarin protocol for heart?

Hi, I'm doing my PhD with mdx animal heart and would like to evaluate with alizarin stain. I found a protocol, but the consistency of the Alizarin was very dense and I think it's not right. Does...

11 June 2021 8,794 0 View

Should I use both LEADING/TRAILING and SLIDINGWINDOW in trimmomatic?

Hello everyone, I have really good quality sequences (shotgun sequencing Novaseq6000) , but I want to filter and trimm them for MAGs assembly. I am using trimmomatic, and I cant achieve that more...

06 April 2021 8,713 1 View

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Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

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Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

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What are the criteria that must be retained for the development and validation of a qualitative NMR method ?

Dear Community, I would like to develop and validate a qualitative NMR method for the analysis of a specific category of chemicals, and my question is the following: What are the criteria that I...

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In what ways do economic factors influence family dynamics and relationships?

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02 July 2024 6,235 0 View

I have NMR,Mass spec,IR and uv of a mixture of three unknown compounds from natural products.I am attaching the data who can help me elucidate?

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