Hi everybody!

I am trying to establish a CTCF ChIP-seq protocol in mouse cells and for this I am testing several antibodies in ChIP-PCR. As a negative control I am looking at the binding of CTCF to the 3'UTR of several genes and also sites that do not display any CTCF binding in published ChIP-seq experiments. Unfortunately the background of my ChIP experiment is really high (% of input of around 3%). I am wondering if you you know any good negative control (site) that I could look at.

Best,

Ekaterini