Hello,
I am looking for some answers regarding the following situation. I am using the GC FID, equipped with a CP SIL 9076 Column to quantify the mono, di and triglycerides from glycerol esters. I observed that in the last period we are unable to detect the triglyceride, form glycerol monooleate (GMO), where normally we have around 10 % of triglycerides. When I have analysed a glycerol trioleate (GTO), the signals for the triglycerides are present. I made some tests, mixing 50% of GMO with 50 % of GTO, and the presence of both GMO and GTO is well represented on the chromatogram. Considering this, I made another mix between 90 % GMO + 10 % GTO. For this last mixture, there is no signal for the presence of GTO. Do you think that the column might be at its end of life? We started to use this column in 2018 and we use it for about 2-3 analyses by week.
Thank you in advance for your time!
Your CP-SIL 9076 column may be near the end of its life after five years of regular use. Degradation of the stationary phase, contamination, or fouling could explain the absence of triglyceride signals in your analyses. Try conditioning the column, trimming 10–15 cm from the inlet, and testing with standards to verify its performance. If issues persist despite these steps, replacing the column is advisable. Ensure the injector and detector are clean to rule out other system-related causes.
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