Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

Universal 16S bacterial primer for feces DNA for qPCR?

I already tested three different sequences and had problems with dimers and melt curve (low specificity). Any reliable primers? I'm studying rat microbiota with qPCR.

12 March 2020 7,658 2 View

Clostridium spp. primer qPCR sequence?

19 January 2020 7,194 3 View

Is Trizol efficient to extract dna from feces (rats)?

I will perform dna extraction from feces and kits are very expensive. I saw that extracting DNA from feces and reading PCR is a bit problematic. Has anyone used any adapted protocol? With lizosima...

07 February 2019 2,225 1 View

How is spatial autocorrelation calculated in niche modeling?

I see the importance of calculating spatial autocorrelation for species occurrence data, in order to guarantee spatial independence. However, I do not understand how this is done. Could any of the...

29 March 2017 8,792 10 View

How do I eliminate negative volumes from the mesh?

16 July 2015 6,044 6 View

Can I analyse 2D-gel (ImageMaster 7.0) of different tissues (leaf vs seed), which are starting the same morphogenetic process? Is this valid?

22 March 2015 4,485 3 View

Do you do a selection before harvesting the supernatant from L929? Which media you use to cultive L929? AlphaMEM or DMEM? And to stock?

16 March 2015 7,058 3 View

Has anyone made electrotransference in Transblot Turbo equipment of BioRad using acrylamide gels 1.5mm?

Hi, we tried to transfer proteins from an acrylamide gel 1.5mm made with our recipe to a PVDF membrane of BioRad in the Transblot Turbo of BioRad, but the majority of protein rested in the gel....

09 February 2015 6,118 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View