18 February 2016 2 2K Report

I've got to do some screening analysis based on MS signals. I calculated molecular masses of revealed compounds. I was wondering if there is some simple on-line search of natural compounds with the use of their masses?

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Does anyone simulate the supercooling phenomenon of the PCM (COMSOL)?

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My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

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Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

16 November 2020 2,373 3 View

How to obtain promoter sequences?

Hi dear fellows, I would like to perform ChIP-PCR on the promoter sequences of genes, but I am not quite sure how to design primers for the promoter sequences. Can someone please help me? My...

20 September 2020 7,371 6 View

Recommended packages or references to combine regional climate models and Holdrige life zones with R?

I want to use R to make regional climate models to analyze changes in Holdridge's life zones. I haven't used R in a long time so I'm kind of outdated and would like to read some references that...

01 July 2020 1,728 4 View

What strategies to use to make distance classes more attractive to students?

In times of pandemic, distance learning has gained strength. What can teachers do to make distance classes more attractive to students?

23 May 2020 8,148 22 View

In your opinion, are bibliographic review articles important and should they be published by scientific journals?

Some journals do not accept review papers because they consider that the theoretical framework is the basis of a research, and not the research itself. Other magazines already accept and encourage...

20 May 2020 7,841 50 View

Metabarcoding fungi in lake sediment cores - advantages/disadvantages of ITS and 18S?

In my PhD, I want to analyse fungal communities in lake sediment cores from Siberia. One of the performed experiments will be a metabarcoding of the fungi. Therefore I am evaluating primers at the...

05 May 2020 3,920 1 View

Which species do you recommend for ecotoxicological tests of industrial effluents containing hydrocarbons?

The question aims to know what types of species are most used in ecotoxicological tests of effluents from industries in the oil sector.

26 April 2020 5,382 21 View

How can I include a second order term in the model?

My hypothesis is that the functional diversity of fish (responsible variable) increases until an optimum level of a gradient of habitat structural complexity (predictor variable), but decreases...

01 April 2020 9,569 4 View

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