I have several soil samples from tropical agricultural soils that i want analysed for Phospholipid Fatty Acid (PLFA) to help study the fungi to bacteria ratio. (It is proposed that PLFA is better for studying the fungi:bacteria ratio compared to next generation sequencing techniques..i hold a contrary opinion). The results of the PLFA will be used alongside those from next gen sequencing (Illumina).
Can anyone reccommend one lab for PLFA in Europe, and preferably Germany?
Also, is it true PLFA gives the best indication for studying fungi to bacteria ratio compared to next generation sequencing techniques?