Hi!

IF we will use a suitable concentration of DNaseI, yes it will diffuse. I want to quantify biomass before and after treatment with DNase! to see the effect of e-DNA.

Thanks

Hi Tamazight,

I don't think DNase I is going to be affected by the dye, especially if you use a buffer as a diluent because this way the enzyme is going to be somehow protected. However, if you want to evaluate the efficacy of a DNase treatment on a biofilm I do not think that applying this enzyme in an already manipulated sample would be the best option since the biofilm wouldn't not be in its "native" state.

My suggestion: You should grow your biofilm samples in the same batch to minimize the variation and then use two different samples (or samples set), one as a control and another one for enzyme testing.

Best of luck with your experiments!

Hi Pedro!

Thank you for answer. You're right, i'll do multiple  samples .

Thanks!