Could anyone help me about sample preparation method for plant crude extract towards GCMS analysis?

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Request for thesis Examiner. Mindfulness, resilience, and wellbeing related topic. Any one interested?

One of my scholars is about to complete a thesis on mindfulness, resilience, and wellbeing. This university requires thesis examiners from different parts of the globe. Should be an associate...

02 June 2024 860 1 View

Cell morphology changes in Invasion assay?

Hi all, I have been working on invasion assay but i have encountered a problem recently. I have attached image below (where one of them showed clear morphology while another are dispersed? Your...

10 January 2024 6,994 0 View

NK-92 Transduction?

Hi all, I will be working on NK92 and may I know if it is better to use retroviral or lentiviral vector if i would like to express a gene in the NK cell cell line? Thanks alot!

31 December 2023 3,276 1 View

How can i write DLL file in TRNSYS 17 after creating my component in proformas?

21 October 2023 3,659 0 View

How can I model regressional analysis for a new high volume fly ash concrete mix in MATLAB AND PYTHON ?

I need how to model and develop mix proportion of HVFAC concrete and regressional analysis also.

30 August 2023 8,246 1 View

How can I get TMY2 data file of a particular place to be able to use in TRNSYS?

That is the weather data of the place is not in my TRNSYS weather library.

12 August 2023 4,865 0 View

Does increased atmospheric humidity affect the hydrogel formation of gelatin methacrylate (GelMA) and silk methacrylate (SilMA) ?

My GelMA samples are not forming proper hydrogels. I used to obtain good hydrogels earlier and I did not change any components from my sample synthesis recipes. The only thing I doubt is that...

10 July 2023 9,144 2 View

Current issues in additive manufacturing?

How can current issues in both conventional and solid-state additive manufacturing processes? What are the various metallurgical solutions available for effectively eradicating interlayer defects...

11 April 2023 7,588 3 View

Trying to synthesize malonyl dichloride from malonic acid using POCl3 but no reaction is observed. What to do to help the reaction take place?

I'm trying to synthesize malonyl dichloride from malonic acid using POCl3 as the chlorinating agent. I'm adding dropwise the POCl3 at 0 Celsius degrees to the solid malonic acid and stirring the...

07 February 2023 7,447 1 View

Does anyone know about which stain to use for negatively charged dipeptide (MW< 1kD) to run in agarose gel?

We want to know the charge of a dipeptide (Mw < 1kD). Dipeptide is based on aspartic acid and its negatively charged. So, to prove that we have carried out 3% agarose gel experiment, tracking...

03 January 2023 5,649 10 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View