What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

Values for Fouling rate in superheaters and economizers in biomass boilers?

I currently work on analyzing fouling rate in BFB biomass boiler, based on available measurements. Looking through literature, the only reported values I found were from certain TEMA Tables, from...

19 April 2020 1,973 5 View

What is the best methodology to use for development of Mobile Applications?

In Software Engineering the UML is considered a standard way in design of the system. What will be the best in designing the mobile applications?

24 January 2020 5,777 3 View

What are the effects of using powerful magnets around AC current?

I am trying to connect two sockets( sockets being flat surfaces on both sides) using powerful (small sized ~8mm diameter) neodymium magnets with 3 or 4 on each side of the socket so that the pull...

03 January 2020 8,835 3 View

How to determine molecular weight of disordered peptides from diffusion NMR data?

From diffusion NMR (DOSY) experiments, diffusion coefficients are available for ~40 AA disordered peptides. Is there a method to calculate the molecular weights using these data, that is suitable...

08 July 2019 703 3 View

Is there a way to blast a protein against all known proteins to find common epitopes? (Even if they are a couple of amino acids long)?

I read somewhere that some vaccinations result in the onset of a different disease that is due to the fact that the vaccine and another protein inside the body have a shared epitope and are both...

22 October 2018 7,832 3 View

What is the best way to differentiate between efferent and afferent sympathetic nerve fibres immunohistologically?

Efferent as afferent renal sympathetic nerve fibers have a important relevance in the development of cardiovascular disease. Thyrosine hydroxylase is a marker for sympathetic fibers. Is there a...

04 June 2018 4,657 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View