I recently isolated RNA from chicken abdominal adipocytes (induced from preadipocytes passaged 5 times). I previously only use cells passaged within 3 times for experiments, and the RNA yield and purity were good. This time, I do not know if it is because the cells were passaged so many times (they are still proliferative) or any other reasons, the 260/280 of some RNA samples were super high (2.2-2.6, or even higher!), which are definitely not right. This situation did not happen with the RNA samples isolated from previous passages of cells. Anyone confronted with such situation before? What could be the possible cause of this?
Hi Utsa,
Thank you very much for your answer. Your suggestion is great, but since my cells are adipocytes, which are very sensitive to the environment change, even the remove of media needs to be done very quickly in case of any breakup of the lipid droplet. I doubt if washing with PBS can cause any instant damage to the cells. Is there anyway else to remove the debris, if that is the case?
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