Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to record RBC autoflourescence in a tissue sample?

RBCs have auto-fluorescence, I want to detect that auto-fluorescence in paraffin embedded tissue samples. Any suggestions?

08 February 2021 9,357 1 View

Why all samples are not detected by RT PCR ?

Hello everyone I did the RT PCR by using cyber green as a fluorescent dye however when I run the RT PCR of my two strains of Saccharomyces cerevisiae (duplicate) then I got the result in which the...

21 November 2020 2,076 3 View

Are there any thumbs up rules or procedures to convert your conference paper to an extended journal version?

Need to write an extended version of my work which is selected to publish in a journal.

28 May 2020 2,607 4 View

I need helper for interpretation of FTIR Graphs, anybody could help?

Can anybody help with interpretation of FTIR Graphs to read peaks of graphs for fruit bars made from fruits, herb and cereal?

18 May 2020 6,159 2 View

How to calculate error of a ratio of two data with errors?

Suppose I need to calculate A =(B/C), where B= 1.00+- 0.33 and C= 1.50+- 0.43 How do I calculate the error of A? as in, A=0.66+- ? Any idea if I can do this in excel or any other...

13 April 2020 7,667 3 View

Have you applied 3D Laser scanning and Building Information Modeling for facility management during operation phase?

14 March 2020 7,806 3 View

I use NPS for drug detection the detection proved by UV, FTIR and AFM but DLS shows a abrupt result how can i interpret the results?

after the interaction of drug the enhancement in the SPR band was observed the intewraction was proved by FTIR and AFM the AFM resiults shows aggregation of NPs the of NPS increases from 40nm to...

10 February 2020 9,355 3 View

Aggregation of nanoparticles leads to large shift in the SPR band but in my case only broadening and quenching in absorption band was observed why?

its colorimetric detection after interacting a analye with nanoparticles color rapidly change from yellow to orange. after 15 min of interaction take of a absorption spectra only quenching...

04 November 2019 7,712 1 View

I want to do protein expression in E. faecalis JH22 by using anhydrotetracycline (ATc) to induce protein expression .Need protocol for expression?

I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.

26 July 2019 1,979 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View

Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

How can I multiply values with standard distribution and with triangular distribution?

I am calculating emissions considering the uncertainty of the data to do Monte Carlo simulation. Some variables such as yields have normal, lognormal or even uniform distributions. These must be...

03 February 2021 6,318 5 View

Matrix based scientific data analysis: extrapolating to matrices of sets?

Data sets, when structured, can be put in vector form (v(1)...v(n)), adding time dependency it's v(i, t) for i=1...n and t=1...T. Then we have a matrix of terms v(i, j)... Matrices are important,...

30 January 2021 9,295 6 View

How to prove possibility/impossibility to analytically solve the functional equation f(a ln x^2)/f(x)=x/2a; and how to find out the solution?

This is my very first encounter with functional equation of this kind, and methods of series solution and differential equations are of not much help. The solution to this problem, or at least the...

25 January 2021 6,053 5 View

Is there anyone who can suggest some related work based on HMM implementation for routing protocol in WSNs?

I'm trying to solve the energy issue conservation in WSNs and I want to use the Markov technique to predict this conservation and demand the routing protocol to select an optimal path among a...

05 January 2021 1,994 2 View