hi
why some plasmid have high copy number and some have low copy number?what controled this copy number?
thank you.Is the copy number depend on host cell?and is RepA in the host genome or plasid genome?
Well my friend, the copy no is controlled by regulating the initiation of plasmid replication. Well in this regulating thing, Rop, a dimer protein which holds the RNA I and RNA II maintains the copy no. RepA also links to the plasmid by binding to their iteron sequence and hence replication is prevented. Basically this process is know as handcuffing.
For RepA it is 1-2 copiers per cell, unless some mutations.(http://www.ncbi.nlm.nih.gov/pubmed/7854325)
And here is an old article, but still useful in understanding some mechanisms that lie in the base of the regulation : http://www.pnas.org/content/82/9/2588.full.pdf
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