I want to transform NIH3T3, D-17 and 293T cells in a packaging cell line transfecting it with a gag-pol vector and a env vector. These will be cotransfected plus my vector of interest. What was the ratio of the 3 vectors that worked better for you?
In a 33 mm well you cannot use (because it is waste) more than 4 ug DNA , scale up
for me, I used the molar ratio of them 1:1:1, you can calculate using their sizes.
Gang Zhang. University of Toronto
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