How to search for suitable journal to publish?

How to search for good journal to publish? I have corrected manuscript in the area of enzymology, looking for a good journal with proper index factor to publish please suggest tq

09 October 2018 3,962 1 View

How to increase the intensity of PCR bands?

I seem to have a huge problem. My PCR bands seems to yield the desired size however they seem to be very faint. Pls help

04 May 2016 5,936 12 View

PUC 19 universal primer

Hi all, I would like to seek yr expert opinion on the universal primers used to sequence PUC19. Is it M13F (-20) and M13R (-26)? The company that i sent to seem to have alot of primers of M13....

08 September 2015 6,927 4 View

Anyone familiar with incomplete digest for RE pET22b?

Hi im doing double digest for pET22b. I used double digest of BamH1 and Hind111 for 1 hr at 37C. The content in (microliter) : Plasmid=25 Buffer = 10 Bamh1= 1 Hind111= 1 AP=2 (alkaline phosphates)

08 September 2015 5,777 8 View

Why transformation grows to the side of the plate?

01 January 1970 4,814 7 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View