Hi, I run 2 replicates for each sample on GC, can I simply use peak area to compare the sample content (higher/lower)? I understand if it needs to do quantitave study, using series calibration curves of standards is required and accurate. Suppose that we dont't want to provide real concentration for each sample, is there any way I can simiply show which content of sample is higher or lower? Thank you for guidance.
Establishing an accurate, valid method using a full calibration table for each compound also establishes the response factor. IOW: Does an increase or decrease in area value translate to a linear or non-linear response relative to the actual concentration amount. With the establishment of a full calibration table (and review of the method to insure it meets good chromatography fundamentals) a determination about what the change in area means would be answered.
The response obtained from a well-maintained GC system is supposed to be positively correlated to the concentration of a certain compound.
However, to get a reliable conclusion, at least the following items should be checked:
(1) Are the matrices of the samples the same;
(2) Is content within the dynamic range of the detector.
hi, to compare two chromatograms, you can choose the percentage integration calculation mode, you will obtain in the result the areas of each peak well based on the retention time
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