The comments from leading researchers around the world were extremely valuable and helpful.
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Hi, does anyone know a good SaCas9 antibody for IHC or immunofluorescence on mouse brain slides? We gave a try with the Epigenetek monoclonal antibody (A-9001) without antigen retrieval (1:500)...
29 December 2020 4,368 5 View
Hi Everybody, I have emulsion adsorbed on graphene surface and would like to get an image using AFM showing both graphene and emulsions. some of my emulsion sizes are 80 nm in diameter, and some...
28 March 2019 4,029 3 View
Hi There, I am using glassy carbon as working electrode and EMIM TFSA as electrolyte, with Pt wire as counter electrode and Ag/AgCl as reference electrode. The CV that I get is really wired. I...
06 November 2018 7,075 3 View
I need good antibodies for alpha1A and alpha1D adrenergic receptors. I've been using one from abcam that is claimed to bind to both subtypes, but it didn't work too well. Moreover, after I've done...
28 September 2018 654 1 View
Hi everybody, I am trying to find an aprotic ionic liquid which is immiscible with certain solvents such as DMF, DMSF, THF, NMP etc. What are the criteria I should be checking in selecting ionic...
18 September 2018 4,656 3 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...
27 February 2021 734 4 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium
24 February 2021 1,915 1 View
Hi I am working recently on a project where I have to study how the thermal properties or heat transfer enhancement of nanofluids as the volume fraction changes, or if a magnetic field is applied...
22 February 2021 961 2 View
Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...
21 February 2021 7,397 1 View
Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...
18 February 2021 3,360 2 View