01 October 2021 1 9K Report

The comments from leading researchers around the world were extremely valuable and helpful.

More Sima Janati's questions See All
Cell culture contamination?

Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...

22 May 2024 8,278 4 View

Which kind of acid is suitable to wash glass equipment?

I need to wash glass ampoules to remove organic residues and other chemical contaminant. Which kind of acid and which concentration of it is needed?

06 January 2024 4,266 3 View

What do two close peaks (doble-peak) in frequency response show us?

I have data from a vibrating specimen in one direction with an input of 1G sine sweep. There are accelerometers in different locations. All data from these sensors in FFT has 2 close peaks. It is...

12 February 2023 9,244 3 View

How can I isolate arthrospira platensis from other contamination?

I want to culture arthrospira platensis sample on agar, but It doesn't make colony and It mix with other contaminant of the sample. How can I isolate a pure spiral?

25 December 2022 5,022 0 View

Can I use sodium hydroxide solid form to absorb the moisture to freeze drying something?

I need to freeze dry cyanobacterial solution, but I don't have acces to freeze dryer. Can I freeze my solution and use "sodium hydrxide solid form" to absorb the moisture?

24 December 2022 4,371 5 View

How to find MicroRNA - gene pathways in bioinformatics?

Hi guys, I want to ask if anyone knows any websites or bioinformatics resources where I can find MicroRNAs or lncRNAs interact with different genes in the shape of a pathway in cancers. I see new...

16 December 2022 4,845 3 View

In micro dilution method for determining the MIC and MBC, why I have problem with my positive control?

I have problem with my positive control well which is my bacteria, it does not change to pink after adding Resazurin dye and incubating for 2 hrs. I repeated the test to make sure I didn’t miss...

07 September 2022 723 0 View

why do I see one single band while I have 2 peaks in qPCR?

Hi all I'm working with qPCR and I persistently find 2 peaks in my qPCR analysis. While my PCR target is 127bps, I see a peak at 82°C and a sharp peak at 88°C. I checked it by agarose...

29 July 2021 9,778 8 View

What is the reason of raising cell culture PH after Adeno virus transduction to Hec cells?

Hi guys, After 72 hours of transduction of Adeno to Hec cells the color of the medium change to purple, cells have been de-attached and many tiny particles are seen. Have you had any experience...

16 July 2021 3,379 1 View

What is best method for total phosphorus measurement by spectrophotometer?

I need to measure total phosphorus in cyanobacteria medium culture

29 June 2021 10,018 7 View

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30 July 2024 4,671 3 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

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Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

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How can we improve transfer learning techniques to make models generalize better across different tasks and domains with limited labeled data?

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Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

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VOC analysis on GCMS ?

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What additional processing or treatment options are available to enhance biogas plant digestate quality or value?

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