Hello
Anyone performed imaging of lysotracker & phalloidin (Alexa Fluor 488) co-localisation?, as classical phalloidin staining has fixation which may not help lysotracker...
appreciate the suggestions
I am synthesizing double-perovskite material. Sr2MgMoO6. After synthesizing, in the calcination process, after 700 degrees Celsius, the sample is sticking on the alumina boat. I tried different...
29 January 2024 3,205 3 View
Hi I'm new to ResearchGate. How do I find all papers that cited me? Thanks, Stan
16 January 2024 4,577 2 View
Hi, we've currently been researching about mutant HIV-1 RT, and we're having a hard time figuring out what software to use in order to determine the protein's binding site (so we wouldn't have to...
24 September 2023 5,723 0 View
I am trying to write a thesis on the relation of the passions of the soul between Aquinas and Avicenna. I am a novice scholar of Arabian philosophical tradition and its relevance in Thomism. Thank...
05 June 2023 7,621 1 View
I am trying to create a data set to help with a statistical research project with my class group? I do not want averages of samples I want raw data. We are researching the effects of outside...
15 November 2022 2,747 0 View
Analyzing for AU on AAS and gain is too hig at 88%. Need to be around 45%
02 August 2022 3,647 3 View
My name is Stanley please help me with the name of the microscope that I can use to view the microalgae structure and able to isolate a single strain.
10 January 2022 9,418 5 View
need to know the concentration calculation for my test
30 October 2021 6,375 3 View
The economic success of north Italian city states was accompanied by great art, which continued or functioned alongside a wealthy Catholic Church. The equal success experienced in the United...
22 October 2020 9,742 10 View
There has been greater concentration on the Medieval Middle Eastern and Central Asian Science but so far insufficient exploration of its roots and what it owes or does not owe to Islam. Few, if...
18 September 2020 1,347 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
We assume that we can find a statistical matrix mechanics equivalent to Schrödinger's PDE in two consecutive steps: i-Transform the Schrödinger PDE describing the wave function Ψ into its square...
27 July 2024 3,959 4 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View