I am culturing induced excitatory neurons. I am getting a lot of clustering of neurons over time. What causes this clustering? Does this clustering and self-adherence reduce the ability of the neurons to adhere to the plate?
Our lab buys 500 mL bottles of RPMI-1640 Medium and stores them at 4C. Oftentimes, for my experiments, I only need about 100 mL total. So, I will make RPMI-1640 + 10% FBS with 90 mL of media and...
22 February 2024 4,544 2 View
I am plating 1 million mouse spleen cells in 96-well plates. In different wells, I am adding different proteins at a concentration of 1 ug/well to try to stimulate T-cell activity. I will then...
18 February 2024 5,121 2 View
I have a low quantity (~1 million) of human natural killer cells that I need to wash several times. These are non-adherent cells in suspension. I am currently spinning these cells down at 300xG...
01 June 2023 7,723 1 View
I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...
07 February 2021 7,860 1 View
I'm confused about what statistical tests would be appropriate to utilise for the following design. I've conducted a questionnaire that measures 5 domains in males and females . I'm interested in...
02 April 2019 3,483 4 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...
09 July 2024 4,001 1 View
Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...
06 July 2024 5,846 1 View
How to modify a neuron in the fully connected layer individually and design it as a statistical indicator such as kurtosis or negative entropy,as shown in the figure
16 June 2024 7,299 0 View
I can understand that we can use Sentaurus TCAD for designing neuron and synapse. But to implement full crossbar architecture using these neuron and synapse for image classification application,...
16 June 2024 6,879 0 View
I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...
12 June 2024 1,714 4 View
Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?
27 May 2024 4,905 3 View