I've been working with a primary endothelial cell line given to us by a collaborator. These cells can only be passaged once, so I grew them to around 70% confluency in a T-75 with no issues. I then passed each flask into (2) 6-well plates, and when I came in today, the media was still its normal light pink color with cloudy swirls. I don't have an image, but it looks like a ton of little floating dots in solution. Under the microscope, they don't seem to move so I don't believe it is microorganisms. I'm fairly positive it isn't the media because I have HUVECs growing in it as well and they're doing fine. This has happened before so I've been even more cautious with my technique so I don't believe that is the issue either. Is anyone familiar with such a thing? I washed the cells and added new media along with some pen/strep to see if it is bacterial. I'll look in a few hours to see if it has made a difference.
If the media is pinkish then I believe it could be yeast contamination. Try to view under microscope to detect any budding characteristics. If it indeed is yeast contamination, you can try adding in AmphotericinB aka Fungizone into the media.
This might be fungal contamination if the media is of normal color as bacteria changes the media to a milky like color.
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